1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
zaharov [31]
3 years ago
14

What are the limitations to using electrophoresis to verify purity of a protein product?

Biology
2 answers:
puteri [66]3 years ago
7 0

Answer:

Use Sodium Dodecylsulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) to analyze the samples you collected in the protein purification experiment. Your final HIC fraction was glowing, so you know you have some GFP. But did any other protein sneak through and affect the purity of your final sample? Run your samples on a gel and find out. If you have other questions about protein purity analysis, the link below may help: <u><em>https://www.creative-proteomics.com/services/purity-analysis-of-peptides-or-proteins.htm</em></u>

Explanation:

9966 [12]3 years ago
6 0
 SDS-PAGE electrophoresis is used to verify the purity of a protein product. Some of the limitations of this method are:
<span><span>·     </span>identification of a band on a protein gel is not always reliable because different polypeptides can have very similar molecular masses,</span>
<span><span>·     </span>band on a protein gel may mask the presence of more than one polypeptide,</span> <span><span>·     </span><span>presence of non-protein residues can affect the mobility of polypeptide and thus cause an error.</span></span>
You might be interested in
What are three three terms used to describe organisms such as cows?
Mrrafil [7]
Heterotrophs, primary consumers, and herbivores..
3 0
3 years ago
What is the equivalent metric liquid volume unit for a cube that measures 10cm in length, 10 cm in width, and 10 cm in height? o
fenix001 [56]

Answer:

1000cm i think

Explanation:

8 0
3 years ago
Read 2 more answers
What human organ can regenerate up to half of itself if damaged?
Wittaler [7]
The liver can regenerate up to half of itself
5 0
2 years ago
Question 11 (1 point)
pishuonlain [190]
A different form of a gene
3 0
3 years ago
1. The cell membrane regulates and controls what kind of<br> move in &amp; out of the cell.
vlabodo [156]

Answer:

The cell membrane is selectively permeable to ions and organic molecules and controls the movement of substances in and out of cells. The basic function of the cell membrane is to protect the cell from its surroundings. It consists of the phospholipid bilayer with embedded proteins.

6 0
3 years ago
Other questions:
  • A mechanism of Darwins proposed theory is
    12·1 answer
  • The mass number of an atom is the same as?
    7·1 answer
  • Imagine you want to identify some organisms in a local pond that you often visit. What key features about such organisms might y
    15·1 answer
  • List<br>A<br>five principles of<br>development<br>growth<br>and​
    12·1 answer
  • Which of the following statements best describes the state of DNA inside of a prokaryotic cell?
    7·1 answer
  • Help if i fail i will not pass
    8·2 answers
  • ATG AATTCTCAATTACCT<br> TACTTAA GAGTTAATGGA<br> How many pieces of DNA would result from this cut
    13·1 answer
  • Autosomal recessive traits require one copy of an allele to be expressed. true or false
    7·1 answer
  • Which of the following processes produces cells that are genetically different from the parent?
    5·1 answer
  • Please help me please find the answer​
    5·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!