Question

What are the limitations to using electrophoresis to verify purity of a protein product?

Answer 1

Answer:

Ｕse Sodium Dodecylsulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) to analyze the samples you collected in the protein purification experiment. Your final HIC fraction was glowing, so you know you have some GFP. But did any other protein sneak through and affect the purity of your final sample? Run your samples on a gel and find out. If you have other questions about protein purity analysis, the link below may help: https://www.creative-proteomics.com/services/purity-analysis-of-peptides-or-proteins.htm

Explanation:

Answer 2

 SDS-PAGE electrophoresis is used to verify the purity of a protein product. Some of the limitations of this method are:
·     identification of a band on a protein gel is not always reliable because different polypeptides can have very similar molecular masses,
·     band on a protein gel may mask the presence of more than one polypeptide, ·     presence of non-protein residues can affect the mobility of polypeptide and thus cause an error.