Protein folding is the physical process by which a protein chain acquires its native 3-dimensional structure, a confirmation that is usually biologically functional, in an expeditious and reproducible manner. It is the physical process by which a polypeptide folds into its characteristic and functional three-dimensional structure from random coil. Each protein exists as an unfolded polypeptide or random coil when translated from a sequence of mRNA to a linear chain of amino acids. This polypeptide lacks any stable (long-lasting) three-dimensional structure (the left-hand side of the first figure). As the polypeptide chain is being synthesized by the ribosome, the linear chain begins to fold into its three-dimensional structure. Folding begins to occur even during translation of the polypeptide chain. Amino acids interact with each other to produce a well-defined three-dimensional structure, the folded protein (the right-hand side of the figure), known as the native state<span>.</span>
Fatty acids when need as a source of energy goes through a process called <span>lipolysis and during this process, it is broken down into a useable energy source. </span>
Answer:
When the solutions (including inside and outside) were replaced by NaCl, the membrane potential changes from a negative value of -60 mV to a positive value of + 60 mV.
Explanation:
According to the Nernst equation the potential of the membrane for a two-compartment model of a cell for positive gradient of K⁺ ions is V = - 60 mVlogK'/K where K' = inside concentration of K⁺ ions and K = outside concentration of K⁺ ions. For a 10 fold excess of KCl in the inside compartment, K'/K = 10. So,
V = - 60 mVlogK'/K = - 60 mVlog10 = -60 mV.
For a negative gradient of Na ions is V = + 60 mVlogK'/K where K' = inside concentration of Na⁺ ions and K = outside concentration of Na⁺ ions. (Since the cell is selectively permeable to Na⁺ ions. So, Na ions to not flow out but in.)For a 10 fold excess of NaCl in the inside compartment, K'/K = 10. So,
V = + 60 mVlogK'/K = + 60 mVlog10 = +60 mV.
So, when the solutions (including inside and outside) were replaced by NaCl, the membrane potential changes from a negative value of -60 mV to a positive value of + 60 mV.
Explanation:
Type II restriction enzymes are the familiar ones used for everyday molecular biology applications such as gene cloning and DNA fragmentation and analysis. These enzymes cleave DNA at fixed positions with respect to their recognition sequence, creating reproducible fragments and distinct gel electrophoresis patterns. Over 3,500 Type II enzymes have been discovered and characterized, recognizing some 350 different DNA sequences. Thousands more ‘putative’ Type II enzymes have been identified by analysis of sequenced bacterial and archaeal genomes, but remain uncharacterized.