Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
exodus 23:8 page 40 and thou shalt take no gift: for the gift blindeth the wise, and perteth the words of the righteous. king james version the holy bible
Explanation:
Answer:
The expectations of the bands will be more on the gels.
Explanation:
One sample provides several sizes and thickness bands in the gel electrophoresis according to the size of fragments that run in it. An individual would expect more bands due to the increase in the number of samples which is 20 samples now.
An increase in the number of samples produces more bands. So one can easily expect more bands from the 20 samples. Scientists would get more bands on the gels.
Thus, the expectations of the bands will be more on the gels.
The answer is True: Any substance can be known by measuring it's density and comparing that density with other substances we already know, so we can give an idea of which group of elements that substance belongs to. It is good to remember that Density (D) is obtained by dividing the mass (M) between volume (V).
For example, when encountering an unknown substance, we can know it's volume by inserting the object into a graduated cylinder, with a known amount of water, and measuring the new volume with the substance in the water; Thus, the mass is divided between the volume, and having then obtained the results, we look for the density of known elements and at the end we can know to which group of elements belongs.
