Simple version:
First, the section with the desirable gene must be identified. Assuming that has already happened, the section of DNA must be excised from the original genome using restriction enzymes, which recognize certain DNA sequences and snip DNA at those sites. DNA ligase is used to "glue" these ends back together. The DNA is inserted into a plasmid (also with restriction enzymes), which would usually contain antibiotic-resistance genes (so they survive in an environment containing the antibiotic, which would also help show if the bacteria have been successfully transformed).
Then comes the actual transformation process. The bacteria to be transformed are mixed with calcium chloride (which causes the bacteria to be more receptive to the plasmids) and then mixed with the plasmids. The bacterial cells are subjected to a heat shock (the solution is heated and rapidly cooled, e.g. by placing the mixture in a hot water bath and quickly transferred to ice) so they will take up the plasmid (since the temperature change makes the membrane more permeable). The bacteria are placed on a growth medium containing the antibiotic they're resistant to. Only those successfully transformed would survive.
Answer:
Option-4
Explanation:
In the given question, S. alterniflora grows in the seaward marsh whereas S. patensis grows in the high marsh. When S. alterniflora is removed, the S. patensis grows to a smaller portion whereas if S. patensis is removed, the S. alterniflora covers all the land.
This shows that although both are different species but they share a niche and the marsh land is the fundamental niche of the S. alterniflora as it can grow in all parts and therefore its distribution is limited by only a realised niche.
Thus, Option-4 is correct.
Answer:
Explanation:
solar energy is harvested as chemical energy in a process that converts water and carbon dioxide to glucose. Oxygen is released as a byproduct. In cellular respiration, oxygen is used to break down glucose, releasing chemical energy and heat in the process.
Answer:
a. midbrain
b. thalamus; hypothalamus, epithalamus
c. cerebral aqueduct
d. medulla oblongata
e. choroid plexus
f. pons; medulla oblongata; midbrain
g. hypothalamus
h. cerebrum
i. epithalamus
j. cerebrum
k. cerebellum
Explanation:
The brainstem is the posterior part of the brain that connects the cerebrum with the spinal cord. The brainstem can be divided into three parts: midbrain (i.e., mesencephalon), the pons (i.e., metencephalon), and the medulla oblongata (i.e., myelencephalon). The mesencephalon is a region of the brain composed of the tectum and tegmentum, which play fundamental roles in motor movement, auditory and visual processing. The corpora quadrigemina is found at the tectum region of the midbrain. The diencephalon is a small part of the brain located above the brainstem (between cerebral hemispheres); which contains the thalamus, hypothalamus, subthalamus, and epithalamus. In turn, the epithalamus is composed of the habenular nuclei, pineal gland, and the stria medullaris thalami. The cerebral aqueduct is a narrow channel (approx. 15 mm) in which the cerebrospinal fluid flows between the third ventricle and the fourth ventricle. The medulla oblongata is a long stem-like structure located in the brainstem of the brain, just in the place where the brainstem connects the brain to the spinal cord. The choroid plexus is a complex network of capillaries located at the cerebral ventricles of the brain, which serve to produce cerebrospinal fluid through ependymal cells that line the ventricles of the brain. The cerebellum is a major structure of the hindbrain and consists of the cerebellar cortex and a core of white matter having the cerebellar nuclei.
Cytoplasm
Ribosomes
Plasmid nucleoid
