You recently joined a lab studying the tyrosine kinase Src, which is present in its normal form and a phosphorylated form. Your
professor wants you to quantify only the phosphorylated version of Src from protein lysate extracted from cells. What assay would you use to quantify the concentration of phosphorylated Sre in the protein lysate? Run through each of the steps in your experiment. (Schematics would be preferred.)
1 answer:
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