The answer is <span>The increased nitrates from the fertilizers leech into the water and cause algal blooms, which take oxygen out of the water and this suffocates fish.</span>.
Algae blooms are the consequence of large amounts of farm
fertilizers in the rivers, which increases nitrates level in the water. Algae use the presence of increased nutrients
and enhance their own growth. It is known that they produce
an oxygen in the photosynthesis, so it is not a problem while they are
still alive. But when these organisms die, their decomposition begins. For the
process of decomposition, the oxygen is needed and ultimately it will be depleted
from a body of water. This could lead to the death of aquatic animals, such as fish, that
depend on oxygen.
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Cooling a substance causes molecules to slow down and get slightly closer together, occupying a smaller volume that results in an increase in density
Heterotrophs are organisms that must consume food from other organisms because they are unable to synthesize their own food molecules.
<h3>What is heterotrophs?</h3>
- An organism is referred to be a heterotroph if it is unable to manufacture food on its own and must obtain it from other sources of organic carbon, primarily plant or animal materials.
- Heterotrophs are primary, secondary, and tertiary consumers in the food chain but not producers.
- Because they eat producers or other consumers, heterotrophs are referred to as consumers.
- Humans, dogs, and birds are all instances of heterotrophs.
- In a food chain, a group of creatures that supply energy and nutrients to other organisms, heterotrophs occupy the second and third levels.
- An organism is referred to as a heterotroph if it consumes other plants or animals for food and energy.
- Its origins are in the Greek words hetero, which means "other," and trophe, which means "nutrition."
- Autotrophs and heterotrophs are two main classifications of organisms depending on how they receive energy and nutrients.
Learn more about heterotrophs here:
brainly.com/question/21450466
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