If a restriction site is altered, a higher molecular weight band will be observed.
Restriction enzymes are proteins synthesized by bacteria to protect itself against foreign DNA. These enzymes cut DNA at specific places.
This enzyme is used to detect mutations in the genome, particularly those at the sites of action of these restriction enzymes. A healthy subject is compared with the patient who is supposed to have cystic fibrosis. If their DNA migration is different, it means that their DNA is different and that there is a mutation at the restriction sites.
sample 1: it has phosphorus, main chemical compund
Answer:
Mainly because if a disorder is dominant, then it will be manifested by all who have the gene. If the disorder is severe, then it will reduce the chances of the individual surviving to grow up and reproduce (unless the disorder only manifests symptoms in later life). If they do reproduce, then 50% of their offspring will inherit the gene, and be at a similar disadvantage in reproduction. Thus, there is likely to be selection against the gene. By contrast, a recessive disorder is only manifested if both parents pass on the abnormal gene, and an individual with one gene for the disorder is usually at no disadvantage. Thus, there is much less selection against abnormal recessive disorders.