Answer:
b. Bacteria
Explanation:
Cell walls with lipopolysaccharide are present in gram Negative bacteria. Gram-negative bacteria have an outer membrane in their cell wall which is composed of lipopolysaccharides.
The lipopolysaccharides are the molecules that have both lipids and proteins. The lipopolysaccharide layer that forms the outer membrane of Gram-negative bacteria is present outside to their thin peptidoglycan layer.
A Negative stain such as India ink or Congo red-Look for the presence of a capsule.
This stain method is usually used to stain the area around the microorganism.
B The Gram stain-Divide bacteria into two groups based on cell wall structure (thick vs. thin).
This is type of differential staining used to distinguish organisms based on their staining properties. Gram + and Gram- bacteria stain different because of different cell wall structure.
C The Ziehl-Neelsen Acid-fast stain- Identify bacteria with waxy cell walls such as: Mycobacterium tuberculosis (the cause of TB).
This is also differential staining method which uses heat and phenol to derive dye into the cells with lipid-rich walls.
D Simple stain with a basic dye-Stain microbes a bright color to make it easier to see them in bright field microscopy.
One dye is used in simple staining in order to determinate the size, shape and arrangement of the cells.
E The Schaeffer-Fulton Endospore stain-Identify Bacillus or Clostridium species, such as the causative agents of anthrax, botulism, tetanus and gangrene.
This is a special type of staining only used for the bacteria that can form endospores. Bacteria are first treated with heat and then with malachite green, which is very strong stain that can penetrate endospores.
The correct answer is C, Oxygen.
During aerobic cellular respiration, oxygen combines with hydrogen ions and water is released as a by-product of respiration.
Explanation;
Cellular respiration allows organisms to release energy stored in chemical bonds of glucose, and other nutrients. The energy in glucose or other nutrients such as fats is used to produce ATP, which cells use to supply their energy needs. During aerobic respiration (in presence of oxygen), oxygen is reduced and water is produced together with carbon dioxide as by-products.
