Hello!
The correct answer is: C. Malleus, Incus, Stapes.
(It's actually C, my bad).
I hope this helped you! c:
The appropriate sequence of steps involved in recombinant DNA technology are as under :-
1) Selected gene is isolated.
2) Restriction enzymes cut DNA into fragments.
3) Fragments of DNA are inserted into a vector.
4) Vector and recombinant DNA multiply.
5) Recombinant DNA is inserted into host.
In the very first step, the desired gene/genetic material for cloning is isolated from the cell. To do this the cell is lysed. Cells can be lysed by sonication or using chemicals like detergents.
In the second step, isolated DNA/gene is cut with the help pf biological scalpels known as restriction enzymes. The vector (usually it is a plasmid) is also cut down using restriction enzymes.
In the third step, the fragment of the restriction digested DNA (gene) and the vector are ligated together.
In the fourth step, this vector in which desired gene has been incorporated is cloned to generate various copies.
In the last step, this recombinant DNA is inserted into a host cell where is expressed i.e. in host cell this desired gene produces its product (protein).
Correct option a) (A) One-quarter will carry the two normal chromosomes, 4 and 12, one-quarter will have only the two translocation chromosomes and no normal chromosomes 4 and 12, and half will have one normal and one trans-located chromosome.
Genetic carrier screening , prenatal screening for aneuploidy and prenatal diagnostic testing have expanded dramatically over the past two decades. The presence of chromosome abnormalities in couples with repeated spontaneous abortion is known even if the phenomenon is far from a complete assessment.
If gene on chromosome or on different chromosomes the recombination frequency 50% . Inheritance of alleles at the two loci are independent.One partner is carries of such balanced translocation have increased risks of infertility , and the chromosomal abnormal offspring.
Genetic counselor should answer to their consultants is to estimate the probability of recurrence of unfavorable pregnancy outcomes among their future pregnancies. The occurrence probability for individual carriers of two different autonomy- Autosome translocation with breakpoints at different positions by stengel- Rutkowaski et.
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