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ivanzaharov [21]
2 years ago
11

The pKa for the side chain of histidine is 6.0. What is the ratio of the deprotonated imidazole side chain to the protonated sid

e chain at pH 5.0 and at pH 7.5?
Biology
1 answer:
Solnce55 [7]2 years ago
7 0

Answer:

When the pKa is 6.0, we can determine the fraction of protonated H is by:

pH = pKa + log [A]/[HA]

Where

A = Deprotonated imidazole side

HA = Protonated side

Given, pH = 5.0

5 = 6 + log [A]/[HA]

log [A]/[HA] = -1 (take antilog of both side)

[A]/[HA] = 0.1

The ratio of the deprotonated imidazole side chain to the protonated side chain at pH 5.0 = 0.1

Given, pH = 7.5

7.5 = 6 + log [A]/[HA]

log [A]/[HA] = 1.5 (take antilog of both sides)

[A]/[HA] = 31.62

The ratio of the deprotonated imidazole side chain to the protonated side chain at pH 5.0 = 31.62

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