Because he was a monk, not a scientist, and his work was not widely published.
B. The recombinant DNA produced by the combination a vector and the gene of interest are inserted in the E. Coli, which produce multiple copies of this recombinant DNA alone with the chromosomal DNA expresses the gene.
During digestion, your pancreas makes pancreatic juices called enzymes. These enzymes break down sugars, fats, and starches. Your pancreas also helps your digestive system by making hormones. These are chemical messengers that travel through your blood.