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Mice21 [21]
3 years ago
8

Why are protein samples treated with sds before they are run on a gel? compare the banding pattern in the prepurification supern

atant lane and the final hic sample lane. what do these results tell you about the success of your purification experiment?
Biology
1 answer:
daser333 [38]3 years ago
4 0
To separate the proteins according to their size, it needs an artifice which involves associating them with molecules of a charged ionic detergent: sodium dodecyl sulfate (or SDS). Via its long hydrocarbon chain, this detergent forms hydrophobic interactions with the peptide chain of the protein and binds at about one molecule of SDS for two amino acids.
The protein-detergent complex thus formed is highly charged, the numerous negative charges of SDS sulfate groups outweighing the few charges carried by the protein. This gives a complex whose total charge is approximately proportional to the length of the peptide chain and thus the electrostatic force exerted during migration in the presence of SDS becomes proportional to the size of the protein.
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Explanation:

A primate is any member of the biological order Primates, the group that contains all the species commonly related to the lemurs, monkeys, and apes, with the latter category including humans. Primates are found all over the world. Non-human primates occur mostly in Central and South America, Africa, and southern Asia

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