Three examples of marine genetically engineered organisms are:
1. AquAdvantage salmon is genetically engineered Atlantic salmon modified in order to achieve fast growth. A growth hormone-regulating gene of Pacific salmon is introduced in the genome of Atlantic salmon.
2. Grass carp was also modified by scientist in order to enhance its protection from contagious diseases. It has been modified with a transgene coding for lactoferrin (antibacterial function). So, the, survival rate of the modified Grass carp was doubled comparing to nonmodifeied fish.
3. Zebrafish is the most commonly used for modification because it has 1-cell embryo, with clear chorion so it is easy to be micro-injected with transgenic DNA.
The microbe 'Virus' causes HIV.
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Answer:
Because of the several steps with high temperatures and also (heavy ) changes in temperature.
Explanation:
PCR consists of a serie of 20–40 repeated temperature changes. Those series or cycles are also called thermal cycles.
The individual steps (common to most PCR methods) are as following:
→Denature template : 99 °C for 2 minutes
→Anneal primers to template : 55 °C for 2 minutes
→Extension of the primers by heat stable DNA polymerase :72 °C for 2 minutes
As we can notice, will in a relatively short time, different temperatures be used. It's important that the used polymerase will be functional at corresponding temperatures, as well as (heavy) temperature changes.
→ Since the bacteria Thermus aquaticus lives near thermal vents in the ocean floor and grows at temperatures of up to 98°C, it's used to corresponding temperatures.
If other enzymes will be used, i.e enzymes functional in lower temperatures (several) steps will not continue or will not be executed properly. To avoid this it's important to use the best temperature resistant enzymes.