The molecules in ice are close together and do not have any movement, or energy at all (frozen). The molecules in water are apart from each other and do have motion and energy. The molecule in gas or vaporization, are far apart from each other, have rapid movement, and have a great amount of energy. Just think that the lighter the liquid state of matter gets, the more energy and motion it has.
In my Biology Honors Class, they taught about gastric juice, or also known as (p H) which is formed by stomach glands, is very acidic and can seriously mess up enzyme trypsin functions. After all, enzymes are a type of catalyst that help break down food, which means that enzymes are a sort of digestive acid as well. Hope this helps!
Answer:
1/8(12.5) if the answer is correct plz mark me as brainliest.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
It depends most packages take 2-3 weeks so I’m guessing about 4 or 5?
