In the absence of autoclave, one can use pressure cooker to sterilize the nutrient agar. The most important thing is that the temperature must be high enough to kill off any microbe present. The pressure cooker can be used in place of autoclave at the temperature of 121 degree Celsius for fifteen minutes.
The Calvin cycle of photosynthesis starts when the energy of light is processed to chemical energy within the cells of plants. The ATP molecules created power the Calvin cycle. The Calvin cycle utilizes energy to create carbohydrates from h20 and co2.