Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
I think that the correct answer to this question is D .
they are both two types of grasslands
<h3><u>Answer;</u></h3>
Lobules
<h3><u>Explanation;</u></h3>
- In respiratory system the lungs are two spongy, cone shaped organs in the thoracic cavity and are separated from each other by the heart and other structures found in the mediastinum.
- Deep grooves called fissures divide each lung into lobes. Each lobe receives its own secondary bronchus. Each lung lobe is divided into smaller segments that are supplied by a tertiary bronchus.
- <em><u>The segments, in turn are subdivided into small compartments called lobules, which contains, lymphatic vessel, arteriole, venules and also a branch from terminal bronchiole wrapped in elastic connective tissue.</u></em>
