Question

Below are steps performed to make an E. coli bacteria that can make human insulin protein for the health care market. List these steps in their proper order.
a. Use heat shock or electroporation to get the recombinant human insulin gene to go into a living E. coli cell.
b. Grow the E. coli in fermentation culture, extract and sell Humulin.
c. Modify the human gene and give it a promoter that will be turned on in E. coli cells.
d. Extract the DNA from a human who does not have diabetes
e. Isolate and clone (make copies of) the gene encoding the insulin protein

Answer 1

Answer:

The following are the steps for making the human insulin protein for the health care market:  

1. Obtain the DNA from a human being who does not have diabetes.  

2. Modify the human gene, and provide it a promoter, which will get turn on when placed within the E.coli cell.  

3. Separate and make copies of the gene encrypting the insulin protein.  

4. Application of electroporation or heat shock method in order to administer the human insulin gene within the living E.coli cell.  

5. Development and growth of E.coli in the fermentation culture, and then extract and sell the humulin in the market.