Answer:
During mitosis, when the nucleus divides, the two chromatids that make up each chromosome separate from each other and move to opposite poles of the cell. ... Mitosis actually occurs in four phases. The phases are called prophase, metaphase, anaphase, and telophase.
Explanation:
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(This is from google)
I believe that the answer is b
Answer:
Juvenile stages of fish and jellfish, small fish, crustaceans and sea stars
Explanation:
hope it helps and give brainliest
By taking daily antibiotics, people are encouraging resistance by getting their immune systems used to the formulas. Antibiotic resistance<span> occurs when bacteria change in a way that reduces the effectiveness of drugs, chemicals, or other agents designed to cure or prevent infections. The bacteria survive and continue to multiply, causing more harm. I think that people can help by not taking antibiotics for every little pain they have, instead try other simple remedies and only use medicines in cases where other remedies won't help. </span>
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR