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alexdok [17]
3 years ago
6

Complete the description of Christian Anfinsen's ribonuclease A renaturation experiment. Anfinsen started with purified ribonucl

ease A in its native state. To begin, he This process resulted in denatured ribonuclease A protein. Next, he which allowed ribonuclease A to return to its native state.
Biology
1 answer:
Sidana [21]3 years ago
5 0

Answer:

In Anfinsen's ribonuclease A renaturation experiment,he wanted to show that protein folding entirely resides within the amino acid sequence of protein.

Explanation:

He used ribonuclease A as a model and removed urea and 2ME from the folding solution.Under these condition, ribonuclease A regains it's biological activity.

This refolding occur in in vitro conditions.

This experiment discover an enzyme called protein disulfide isomerase(PDI) which catalyze the reduction of incorrect dissulfide bonds.This also helps in trapping protein in an incorrect conformationso that it can un fold and try agin.

This PDI plays important role in proper folding,and the active site of this enzyme have disulfide.

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Transcription is the biological process where RNA is formed. As you may already know, RNA is an extremely important nucleic acid for genetic processes and the production of proteins. The transcription has three steps that are called start, elongation and end. In the elongation phase, the RNA strand is growing, at this time, RNA polymerase, which is the enzyme responsible for RNA elaboration, places the nucleotides, in the RNA strand, in the 5' - 3' direction. This enzyme causes the hydroxyl, which makes up the nucleotide at the 3' end of the forming RNA, to attack the phosphorus present in the incoming  ribonucleotide.

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