Nicked circle is the reason.
Explanation:
During recombinant DNA technology the gene of interest is ligated to plasmid DNA by excision with molecular scissors or endonuclease enzyme and ligated with enzyme ligase.
Enzyme ligase catalysed by ATP seals the nicks by making phosphodiester bonds in the ds or ss DNA and release of ADP.
The ligation in the plasmid did not occur properly hence the replication is interrupted hence no new plasmids with gene of interest replicated in the ligation carried out.
the way to tell is to lay one(or both) of the wings down flat. turn them so that the bumpier sides are facing up(the bumps are from the feathers being pulled from the follicles) If it is a full wing(drumstick and wingette,) there should be a pointed end. if you face the drumstick nub(handle, bone, whatever you call it,) inward, you can tell... it will be pretty obvious. if the nub is facing the left, it is the right wing and vice versa
Answer:
The beta globin protein is one of the subunits of hemoglobin, a protein necessary for the oxygen-carrying function of red blood cells. People with the sickle cell mutation in both copies of the HBB gene produce proteins that clump together and lead to changes in the shape and behavior of red blood cells.
Answer: (Smallest = left) (Largest = right)
Individual = community = population = biome = biosphere = ecosystem
The answer is “the sun exerts gravitational force onto the planets” hope this helps you :)
