Question

Order the steps required to analyze gene expression from a particular cell type using a dna microarray.

Answer 1

This is the order;

1.       Extract mRNA from cells

2.       Reverse transcribe mRNA to cDNA

3.       Label cDNA with a chemiluminescent molecule

4.       Add cDNA to microarray and incubate

5.       Wash away unbound cDNA

6.       Visualize microarray and analyze the data