Non polar molecule move across a membrane through simple diffusion, when moving up their concentration gradient. Simple diffusion involves movement of molecules through a membrane without the help of integral membrane protein. These molecules are driven by the force of diffusion. This is different from facilitated diffusion where molecules only move with the aid of integral protein in the membrane.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
true
Explanation:
mutations deal with chromosomal changes, and while they might not always be reflected in the phenotype.. the genotype does change
<span>It may be safe to say that we can rule out a concussion for this patient. The CT scan is negative for trauma to the skull, brain bleed and negative for tumors. However, there is a slight abnormality regarding the smaller size of the ventricles but otherwise unremarkable.</span>
