<span>The most crucial principle of ELISA (enzyme-linked immunosorbent assay) is a highly specific antibody-antigen interaction.</span>
ELISA is a biochemical technique used to detect the presence of an antibody or an antigen in the biological sample. Simply described, in an ELISA, an antigen is immobilized on a solid surface and then a specific antibody is applied over the surface so that it can bind to the antigen. The antibody is usually linked to an enzyme, and in the final step, a substrate for that enzyme is added. The enzyme can convert it to some detectable signal, most commonly a color change. <span>Medical usage of ELISA is in the diagnosis of HIV infection, pregnancy tests, measurement of cytokines…</span>
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Answer:
Mountains are cooler than plains because with increase in height the temperature decreases. EXPLANATION: Mountains are located in high elevations and plains are located in low elevations. Their respective heights are measured by term called as their height above sea level.
