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professor190 [17]
3 years ago
11

What is the purpose of the lab?

Biology
2 answers:
erica [24]3 years ago
8 0

Answer:

First, observed all of the arthropods under a magnifying glass and took notes on the legs, antennae, stingers, claws, and wings.

Then, I selected a question out of the key they gave me and started with, "Does the arthropod have a stinger?".

Next, I started to sort the arthropods into yes and no branches coming off of my question.

After that, I chose another question for my yes group that went with some of the organisms and not with the others. My question was, "Does the arthropod have antennae?" because some did and the others didn't.

I kept continuing this process and got to where all of the arthropods were separated into their own groups and checked over the dichotomous key to make sure all of the questions and answers were correct.

Explanation:

Alex787 [66]3 years ago
5 0

Explanation:

Accordingly, the introductory physics laboratories have the following purposes and goals:

1. To provide an experimental foundation for the theoretical concepts introduced in the lectures. It is important that students have an opportunity to verify some of the ideas for themselves.

2. To familiarize students with experimental apparatus, the scientific method, and methods of data analysis so that they will have some idea of the inductive process by which the ideas were originated. To teach how to make careful experimental observations and how to think about and draw conclusions from such data.

3. To introduce the methods used for estimating and dealing with experimental uncertainties, including simple ideas in probability theory and the distinctions between random (statistical) and systematic "errors." This is essential in understanding what valid conclusions can be deduced from experimental data and that, properly obtained, these conclusions are valid, notwithstanding the uncertainty of the data.

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Answer:

Given:

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Amount of DNA used for transformation = 100ng

Volume of competent cells used = 100ul

Total volume = 1ml (1000ul)

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The amount of cells transformed per 1 µg of DNA is called the transformation efficiency

(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA

Amount of DNA used for plating = 100ng * (200/1000)

                                                      = 100ng * (1/5)

                                                      = 100/5 = 20ng

Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng

               (2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA

Transformation efficiency = 2000 Transformants / ug DNA

                                          = 2 x 10³ Transformants / ug DNA

Explanation:

Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.

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