IV only
Plate IV will only have the trangenic E Coli hence will have the highest percentage of bacteria that are expected to produce insulin.
Explanation:
The transgenic <em>E. coli </em>will have taken up the plasmid with the insulin and <em>amp</em> genes hence they will be able to survive and grow in amplicilin antibiotic.
In plate IV, the wild type <em>E. coli</em> will be eliminated by the antibiotic (because they do not have the gene for resistance) hence providing no competition for resources, in the agar, with the transgenic <em>E coli</em>. This will ensure optimal growth for the transgenic <em>E coli</em> even, than in plate III, producing highest percentage of insulin.
Learn More:
For more on gene cloning check out;
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