Question

in a second experiment, the plasmid contained the gene for human insulin as well as the amp gene. which of the following best plates would have the highest percentage of bacteria that are expected to produce insulin?

Answer 1

IV only

Plate IV will only have the trangenic E Coli hence will have the highest percentage of bacteria that are expected to produce insulin.

Explanation:

The transgenic E. coli will have taken up the plasmid with the insulin and amp genes hence they will be able to survive and grow in amplicilin antibiotic.

In plate IV, the wild type E. coli will be eliminated by the antibiotic (because they do not have the gene for resistance) hence providing no competition for resources, in the agar, with the transgenic E coli. This will ensure optimal growth for the transgenic E coli even, than in plate III, producing highest percentage of insulin.

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