CDNA does not have introns and therefore can be expressed in prokaryotic cells. cDNA libraries are most useful in reverse genetics where the additional genomic information is less use. Also, it is useful for subsequently isolating the gene that codes for that mRNA.
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Answer:
In many cases, cloning involves inserting the gene into a piece of circular DNA called a plasmid, which can be copied in bacteria. ... If two DNA molecules have matching ends, they can be joined by the enzyme DNA ligase. DNA ligase seals the gap between the molecules, forming a single piece of DNA.
A frame-shift mutation would occur. This is because a
deletion will cause a change in codon sequence from the point of mutation to
the end of the mRNA. If the mutation is
closer to the start of the mRNA, the larger the effect of the mutation on the
translated protein.