<h2>CRISPR/Cas9</h2>
Explanation:
CRISPR can be used to reintroduce dystrophin back into the KO mouse
- CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats and is used to for gene editing
- CRISPR/Cas-mediated genome editing has been shown to permanently correct DMD mutations and restore dystrophin function in mouse models
- Germline editing by injecting zygotes with CRISPR/Cas9 editing component was first done in mdx mice by correcting the mutated exon 23
- Postnatal editing of mdx mice was then achieved using recombinant adeno-associated virus to deliver CRISPR/Cas9 genome editing components and correct the dystrophin gene by skipping or deleting the mutated exon 23 in vivo
- Germline and postnatal CRISPR/Cas9 editing approaches both successfully restored dystrophin function in the mice and same technique can be used for KO mouse model
The last one, wind blowing for a long time wont cause much wind, but if the wind is short and choppy, it will cause the waves to be choppy.
Answer:
Other species that rely on those fish would die off. The extinction of one species can lead to the extinction of another and so on.
<u>Answer</u>:
1. Type of medium - the nutrient availability is one of the vital factors for the growth of a bacterial colony. Thus, the used growth medium will fulfill this requirement.
2. Incubation temperature - all bacteria have an optimal temperature range in which maximum growth is achieved. Any variations from this will result in negative effects on the amount of bacteria that are produced.
3. Moisture - bacteria thrive in moist or wet environments. Thus, the availability of moisture is another vital factor for the growth of the colony.
