Answer:
What is the effect of power/magnification on the frequency and size of organelles under a microscope?
Explanation:
Organelles within the cell are responsible for carrying out various functions. Some cells are more specialized than others, and may have particular organelles at a higher frequency, or showing a variation in size; sub-cellular structures become more visible at higher magnifications under the microscope.
Hypotheses:
- organelle A's frequency decreases while B's frequency increases at higher magnifications
- organelle A's size increases while B's size decreases at higher magnifications
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<em>Dependent variables: size and frequency cell organelles</em>
<em>Independent variable: power/magnification at low (x4), medium(x10) and high (x40)</em>
<em>Controlled variables: Type of organelles, microscope used, cell examined, </em>
Method:
1. Examine the organelles A and B in a cell mounted on a slide; use the fine adjustment to focus on the cell.
- Frequency: What is the average number of organelle A versus B, seen at low (x4), medium (x10) and high (x40) magnifications?
- Size: Measure the average diameter of organelle A versus B using an ocular micrometer at low, medium and high magnification.
2. Record and tabulate observations.
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Answer:
DNA is negatively charged. When an electrical current is passed through a gel, the DNA is repelled by the nearby negative current and pushed across the gel towards the positively charged side.
Explanation:
Electrophoresis has become a standard molecular biology technique. This technique is based on the use of a constant-uniform electric field that enables the separation and identification of DNA fragments with different lengths, which form bands in the electrophoresis gel. Electrophoresis can be used to analyze not only DNA, but also RNA and proteins. During electrophoresis, the fragments of DNA that are negatively charged will migrate towards the positively charged electrode, where the duration of this movement will depend on the size of the DNA fragment, as well as of the pore size in the gel.
