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lilavasa [31]
3 years ago
12

What are rod-shaped and spore-forming bacteria known as?

Biology
1 answer:
Alexus [3.1K]3 years ago
6 0
They are known as Bacillus
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1. At what pH do the following enzymes work best?<br> Suerase:<br> b. Trypsin:
tekilochka [14]

Answer:

1.7

b.Trypsin is an enzyme that helps us digest protein. In the small intestine, trypsin breaks down proteins, continuing the process of digestion that began in the stomach. It may also be referred to as a proteolytic enzyme, or proteinase. Trypsin is produced by the pancreas in an inactive form called trypsinogen.Apr 13, 2018

Explanation:

3 0
2 years ago
A DNA fragment is introduced into the lacZ gene of a plasmid, which also contains an ampicillin resistance gene. What is the app
zhenek [66]

Answer: The bacteria transformed with this particular plasmid will form white colonies on the plates containing ampicillin and Xgal.

Explanation: The lacZ gene produces an enzyme called β-galactosidase which is responsible for the breakdown of lactose into glucose and galactose. The lacZ gene is one of the three genes (the other two being lacA and lacY) of the lac operon which is responsible for the transport and mechanism of lactose in E. coli and many other bacteria.

In recombinant DNA technology, when a plasmid is to be used to transform a host cell, such markers are used to help screen the transformed cells from the ones that have not taken up the plasmid. Xgal present in the plates is an artificial substrate which is hydrolyzed by

β-galactosidase into 5-bromo-4-chloro-indoxyl which will dimerize and oxidise into 5,5'-dibromo-4,4'dichloro-indigo. This is a blue pigment which will give blue color to the bacterial cells. Introducing a DNA fragment in this lacZ gene will make it non-functional so it will not be able to produce the enzyme.

Therefore, when a bacterial cell is transformed with a plasmid containing ampicillin resistance gene and a DNA fragment introduced in the lacZ gene and then grown on plates containing ampicillin and Xgal, white colored colonies will appear. The white colonies will show the bacterial cells that have successfully taken up the plasmid with the DNA fragment incorporated in the lacZ gene as this will render the gene non-functional and will not produce β-galactosidase which will breakdown Xgal to give blue colonies. Since the plates contain ampicillin, only the bacterial cells that have been successfully transformed with the plasmid ( the ones that have the DNA fragment and the ones without it) will grow as the ampicillin resistance will give them resistance against ampicillin in the plates. The bacterial cells that have not taken up the plasmid will not be resistant to ampicillin and will not form colonies on the plate.

This is called blue-white screening which is used to identify successfully transformed host cells. A picture of this is given in the attachment, taken from the following website:

https://www.mun.ca/biology/scarr/Blue_&_White_Colonies.html

8 0
3 years ago
The main function of organelles is
ZanzabumX [31]

Answer:

3 option idk

Explanation:

3 0
3 years ago
Explain the light-dependent and light-independent reactions that occur in photosynthesis. plz, help brainliest for best. not mul
yKpoI14uk [10]

Answer:

In the light-independent reactions or Calvin cycle, the energized electrons from the light-dependent reactions provide the energy to form carbohydrates from carbon dioxide molecules.  

Explanation:

After the energy is transferred, the energy carrier molecules return to the light-dependent reactions to obtain more energized electrons.

3 0
2 years ago
What is the smallest part of an element that retains the properties of the element?
anyanavicka [17]

Answer:atom

Explanation:because it is defined as the smallest form of any given elemental item

6 0
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