When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
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Answer:
Structurally, plant and animal cells are very similar because they are both eukaryotic cells. They both contain membrane-bound organelles such as the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes, and peroxisomes. Both also contain similar membranes, cytosol, and cytoskeletal element.
The mechanism of <u>coagulation </u>involves the activation, adhesion, and aggregation of platelets to cause blood clotting.
1.
B
Photosynthetic organisms require light, in which the sun’s rays can only go so far
2.
C
The energy flow pyramid goes from decomposers to producers, meaning that if producers are dying out due to lack of nutrients, there must be something wrong with the decomposer population
