Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
Answer:
A blizzard can form when warm air combines with a high pressure system.
Explanation:
A high pressure zone is defined by cold air mass that has higher density than surrounding air mass. Therefore when a warm air current blows towards the high pressure system, the warm air mass rises over the cold air mass of the high pressure system.
The warm air begins to cool at the front between the cold air mass and warm air mass. Usually for a blizzard to occur the cold air mass is usually below freezing point temperatures. Therefore the moisture in the warm air cools and freezes into snowflakes. This is why the winds of a blizzard are accompanied by snowflakes akin to a winter storm.
Lean More:
For more on blizzards check out;
brainly.com/question/2852460
brainly.com/question/11961252
#LearnWithBrainly
Because their jobs required them to set all day.
The Nurse should include to the instruction is to drink a
full glass of water with each dose and at least 8 more glasses (8 ounces each)
of fluid a day or drink 3000 mL a day. . If your doctor has directed you to
drink less fluid for other medical reasons, Dosage is based on your medical
condition and response to treatment.
Answer:
moth is biotic
Explanation:
biotics are living things
