Question

Predict the most likely outcome of culturing transformed E. coli in the presence of tetracycline.Justify your prediction.

Answer 1

Answer:

E.coli is a bacterium which is preffered for transformation due to its rapid growth and high efficiency of introduction of DNA molecules into cells.

Tetracycline is an antibiotic used to treat several infections such as cholera, plague, brucellosis, and malaria etcetra.

Plasmid is a small, extrachromosomal DNA molecule present in cell which is seprated from chromosomal DNA and have ability to replicate independently.

When a plasmid resistant to tetracycline and kanamycine is inserted into E.coli, the process is called transformation. E.coli might not be able to survive due to absence of plasmid but the transformed strains having antibiotic tetracycline and kanamycine will probably be able to survive it.

Hence, the culture of bacteria carrying plasmid will be able to survive only.