When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:NADH donates it electron to complex I a higher energy level than other complexes while FADH donates it electron to complex II a lower energy complex.
Explanation:
Both NADH and FADH are shuttle of high energy electrons originally extracted from food into the inner mitochondrial membrane.
NADH donate it electron to a flavoprotein consisting of FMN prosthetic group and an iron-sulphur protein in ETC complex-I. Two electrons and one hydrogen ion are are transferred from NADH to the flavin prosthetic group of the enzyme.
While the electrons from FADH2 enters the ETC (electron transport chain) at the level of co-enzyme Q (complex II). This step does not librate enough energy to act as a proton pump.
So NADH produces 2.5 ATP during the ETC and oxidative phosphorylation because it donates its electron to Complex I, which pump more electrons across the membrane than other complexes.
Pangea was the supercontinent that had all the landmasses of earth together before the Triassic period and that broke up into Laurasia and Gondwanan. However, Panthalassa is a prehistoric ocean that was surrounding Pangea.
Hope this helps!
Two scientists testing different hypotheses about the same question
