Extraction, homogenization, and centrifugation are the three processes in cell fractionation.
Cell fractionation is a method for dividing cellular components while yet maintaining each component's unique functionality. This technique was initially applied to show where distinct biochemical activities are located within cells. Subcellular fractionation is also used to help with illness detection and to offer an enhanced source of protein for further purification. To prevent osmotic damage, tissue is routinely homogenized in an isotonic buffer solution. Grinding, mincing, chopping, pressure changes, osmotic shock, freeze-thawing, and ultrasonic waves are a few of the mechanisms for homogenization. After that, the samples are maintained cool to avoid enzymatic deterioration. It is the development of a uniform mass of cells (cell homogenate or cell suspension).
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Explanation:
B. Lactic acid is produced from lactobacilli as the starter undergoes fermentation
Thus, they utilize other means for the generation of energy in the form of ATP and to replenish NAD+ an oxidized form of NADH, the main electron carrier in glycolysis. Pyruvate (pyruvic acid) is produced in the cytoplasm via glycolysis- it is also used as an electron acceptor in a process called fermentation. In lactic acid bacteria, the pyruvate produced is directly transferred to lactate (a form of lactic acid) producing NAD+.
Further Explanation:
In all eukaryotic cells mitochondria are small cellular organelles bound by membranes, these make most of the chemical energy required for powering the biochemical reactions within the cell. This chemical energy is stored within the molecule ATP which is produced. Respiration in the mitochondria utilizes oxygen for the production of ATP in the Krebs’ or Citric acid cycle via the oxidization of pyruvate( through the process of glycolysis in the cytoplasm).
Oxidative phosphorylation describes a process in which the NADH and FADH2 made in previous steps of respiration process give up electrons in the electron transport chain these are converted it to their previous forms, NADH+ and FAD. Electrons continue to move down the chain the energy they release is used in pumping protons out of the matrix of the mitochondria.
This forms a gradient where there is a differential in the number of protons on either side of the membrane the protons flow or re-enter the matrix through the enzyme ATP synthase, which makes the energy storage molecules of ATP from the reduction of ADP. At the end of the electron transport, three molecules of oxygen accept electrons and protons to form molecules of water...
- Glycolysis: occurs in the cytoplasm 2 molecules of ATP are used to cleave glucose into 2 pyruvates, 4 ATP and 2 electron carrying NADH molecules. (2 ATP are utilized for a net ATP of 2)
- The Citric acid or Kreb's cycle: in the mitochondrial matrix- 6 molecules of CO2 are produced by combining oxygen and the carbon within pyruvate, 2 ATP oxygen molecules, 8 NADH and 2 FADH2.
- The electron transport chain, ETC: in the inner mitochondrial membrane, 34 ATP, electrons combine with H+ split from 10 NADH, 4 FADH2, renewing the number of electron acceptors and 3 oxygen; this forms 6 H2O, 10 NAD+, 4 FAD.
Within cells, aerobic respiration may not occur due to several factors:
- - a lack of inorganic, final electron acceptors
- -incomplete or lack of a complete electron transport system
- -missing genes for enzymes within the Kreb's cycle
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The answer is C
groundwater is contained and flows through bodies of rock and sediment called aquifers. The amount of time that groundwater remains in aquifers is called its residence time, which can vary widely, from a few days or weeks to 10 thousand years or more.
A statistic used in the fields of breeding and genetics that estimates the degree of variation in a phenotypic trait in a population that is due to genetic variation between individuals in that population.
ATP contains energy in the chemical bonds between its phosphate groups, <span>best explains how the structure of ATP helps provide energy to the cell.</span>