If one wishes to clone a gene using typical restriction endonucleases, how does the restriction endonuclease identify the appropriate cut sites in the genome using the palindromic sequence.
Restriction endonucleases are a family of enzymes usually found in bacteria that cuts DNA at a specific sequence called recognition site. There are three type of restriction endonuclease Type I, Type II, and Type III.
Each endonuclease recognizes a particular sequence called palindromic sequence which is a sequence that reads the same sequence in both the strands either reading from the 3 prime end or 5 prime end.
The endonuclease cuts the palindromic sequence in such a way that it produces sticky ends.
To learn more about restriction endonucleases here
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Presence of a teratogen in the body of mother during pregnancy is responsible for mal growth of child or incomplete growth which may lead to abortion, still birth or even a defective child.
<h3><u>Explanation:</u></h3>
A teratogen is a substance that is known to cause <em>defects</em> in growth of child during the intrauterine growth period. Thalidomide was used as a morning sickness drug which relieved the vomiting nature of the would be mother. But the presence of thalidomide was responsible for less growth of limbs of the child. Alcohol in intrauterine life may cause Fetal Alcoholic Syndrome which damages the fetus' sense organs and neural cord.
The presence of a teratogen during the 1st trimester is more serious than in last trimester because the main formation of different organs and systems take place in the 1st trimester and maturity takes place in third trimester.
Difficult to change - nutrients! crops won’t grow on soil that has had all of the nutrients used up. example: after growing corn in the same place for 50 years, corn will not grow anymore because all of the nutrients it needs to grow will no longer be in the soil
