Passive transport:
For simple diffusion = fatty acids, amino acids, steroid hormones (because they’re non-polar)
Facilitated diffusion = N2 and Cl-
Because mutations do not always result in an amino acid change.
Whatever you do do not click on that link
D.)The enzyme changes shape and no longer fits the substrate.
High temp causes enzymes to speed up the reaction but too much heat and the enzyme might denature, losing its shape.