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Vladimir [108]
3 years ago
12

You are a researcher with an interest in understanding the mechanism of serine protease enzymes. To test the effect of the role

of Asp102 in the efficiency of catalysis, you make targeted substitutions of that residue and compare the rate of hydrolysis of p-nitrophenyl acetate with the rate on the unmutated enzyme. Answer the following questions: a. (4) You observe a bi-phasic plot, i.e. a quick burst of product (p-nitrophenol) release (seen as a steep curve), followed by a slower (less steep) curve. What step of the reaction corresponds to each phase of the chymotrypsin reaction? And which corresponds to the Michaelis-Menten Steady State? Explain. b. (4) If you mutate Asp102 to create an enzyme with Asn102, describe (or draw) changes you would expect to see in p-nitrophenol release – and, of course, explain your answer. c. (4-8) For the wild type (unmutated) enzyme, you measure a rate of p-nitrophenol release by the change in absorbance at 405 nm (for the slower phase only) of 0.216 (in a cuvette with a pathlength of 1 cm) over the course of two minutes. Use the Beer-Lambert Law to calculate the concentration of product released over this time; the extinction coefficient of p-nitrophenol at 405 nm is 18,000 M-1 cm-1. For extra credit: If your assay is done with 1 x 10-9 M enzyme, what is the turnover number for this enzyme?
Chemistry
1 answer:
yawa3891 [41]3 years ago
5 0
Ngl didn’t read it so I’m not gonna answer it. Don’t report please just want to ask a question but have to answer two
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Determina el numero de masa para el paladio en la siguiente tabla:*
Damm [24]

The concept of atomic masses we can find that the correct answer is:

       c) 101

The atomic mass or mass number of an element is the sum of its protons plus the neutrons that are in the atomic nucleus, the electrons are not taken into account because they have a mass much lower than the masses of protons and neutrons.

   

In the table they indicate that there are 46 protons and 55 neutrons, therefore

          M = #_ {protons} + #_ {neutrons}

          M = 46 +55

          M = 101

In conclusion using the concept of atomic masses we find that the correct answer is:

c) 101

Learn more about atomic mass here:

brainly.com/question/1317964

5 0
2 years ago
The light reactions of photosynthesis use _____ and produce _____. The light reactions of photosynthesis use _____ and produce _
SashulF [63]
<h2>Input = NADP^+, water and Output = NADPH + O_2</h2>

Explanation:

The light reactions of photosynthesis use water and produce Oxygen, NADPH.

The equation for photosynthesis :

6 CO_2 + 6 H_2O → C_6H_12O_6 + 6 O_2

The process of photosynthesis in two stages -

  • The first stage is called the light reaction in which the light energy from the sun is captured and converted into chemical energy stored in the form of ATP and NADPH
  • The second stage is the process of conversion of ATP molecules to sugar or glucose (the Calvin Cycle)

For a light reaction -

Net Input is of, NADP^+, Light, Water, ADP

Net Output is of, ATP, NADPH, O_2

8 0
3 years ago
What is the pressure in a 245 L tank that contains 5.21 kg of helium at 27 Celsius ​
Kitty [74]
<h2><u>Answer:</u></h2>

(These are not rounded to the correct decimal)

130.94 atm

13,266.6 kPa

99,571.4 mmHg

<h2><u>Explanation:</u></h2>

<u></u>

PV = nRT

V = 245L

P = ?

R = 0.08206 (atm) , 8.314 (kPa) , 62.4 (mmHg)

T = 273.15 + 27 = 300.15K

n = 1302.5 moles

How I found (n).

5.21kg x 1000g/1kg x 1 mole/4.0g = 1302.5 moles

Now, plug all the numbers into the equation.

Pressure in atm = (1302.5)(0.08206)(300.15) / 245 = 130.94 atm (not rounded to the correct decimal)

Pressure in kPa = (1302.5)(8.314)(300.15) / 245 = 13,266.6 kPa (not rounded to the correct decimal)

Pressure in mmHg = (1302.5)(62.4)(300.15) / 245 = 99,571.4 mmHg (not rounded to the correct decimal)

5 0
3 years ago
What charge does a proton have
Nata [24]
It has a positive charge of 1
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Day/Night and Seasons (8.7A)
Natasha_Volkova [10]
The length of the seasons would increase
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