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Vladimir [108]
3 years ago
12

You are a researcher with an interest in understanding the mechanism of serine protease enzymes. To test the effect of the role

of Asp102 in the efficiency of catalysis, you make targeted substitutions of that residue and compare the rate of hydrolysis of p-nitrophenyl acetate with the rate on the unmutated enzyme. Answer the following questions: a. (4) You observe a bi-phasic plot, i.e. a quick burst of product (p-nitrophenol) release (seen as a steep curve), followed by a slower (less steep) curve. What step of the reaction corresponds to each phase of the chymotrypsin reaction? And which corresponds to the Michaelis-Menten Steady State? Explain. b. (4) If you mutate Asp102 to create an enzyme with Asn102, describe (or draw) changes you would expect to see in p-nitrophenol release – and, of course, explain your answer. c. (4-8) For the wild type (unmutated) enzyme, you measure a rate of p-nitrophenol release by the change in absorbance at 405 nm (for the slower phase only) of 0.216 (in a cuvette with a pathlength of 1 cm) over the course of two minutes. Use the Beer-Lambert Law to calculate the concentration of product released over this time; the extinction coefficient of p-nitrophenol at 405 nm is 18,000 M-1 cm-1. For extra credit: If your assay is done with 1 x 10-9 M enzyme, what is the turnover number for this enzyme?
Chemistry
1 answer:
yawa3891 [41]3 years ago
5 0
Ngl didn’t read it so I’m not gonna answer it. Don’t report please just want to ask a question but have to answer two
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Either he’s busy, or he is just trying to ignore you.

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7 0
3 years ago
Does a displacement reaction take place in 'magnesium + lead nitrate'? and if so why?
cestrela7 [59]

Answer:

Maybe or maybe not (not sure)

Explanation:

A displacement reaction is a type of reaction where one element is displaced by another from a compound.

In the case of magnesium and lead nitrate, magnesium is more reactive than lead. Therefore, it will displace lead from lead nitrate to form magnesium nitrate and lead.

The reaction can be represented as:

Mg(s) + Pb(NO3)2(aq) → Mg(NO3)2(aq) + Pb(s)


Another answer could be;

A displacement reaction does not take place in 'magnesium + lead nitrate' because magnesium is more reactive than lead.

5 0
2 years ago
Match each element with the element that is most similar to it. If you don’t know the answer, use this periodic table to help yo
yan [13]

Answer:

Lithium - Potassium

Carbon - Silicon

Fluorine - Bromine

Oxygen - Sulfur

Explanation:

I hope this helps!! :3

5 0
3 years ago
Consider the reaction below. NH4+ + H2O mc013-1.jpg NH3 + H3O+ Which is an acid-conjugate base pair? NH4+ and NH3 NH4+ and H3O+
juin [17]
NH4+ and NH3 are an acid-conjugate base pair, since NH4+ is an acid, while NH3 is its conjugate base (since it is without the H+).
H2O and H3O+ can also be considered an acid-conjugate base pair, since H3O+ is an acid, while H2O would be its conjugate base. (But if only 1 answer is to be selected, it should be the NH4+ and NH3)
NH4+ and H3O+ are both acids, and both H2O and NH3 can be considered bases.
4 0
3 years ago
Read 2 more answers
Dimensional analysis can only be used for time, distance, mass, speed, volume, and chemical quantities.
cupoosta [38]

Dimensional analysis can only be used for time, distance, mass, speed, volume, and chemical quantities: False.

<h3>What is dimensional analysis?</h3>

A dimensional analysis can be defined as an analysis of the relationships that exist between different physical quantities by identifying their fundamental (base) quantities, especially for the purpose of inferences.

Dimensional analysis is also referred to as unit-factor method or factor-label method and it is typically used to convert a different type of unit to another.

Generally, dimensional analysis can only be used for the following physical quantities:

  • Time
  • Distance
  • Mass
  • Volume
  • Speed

In conclusion, a dimensional analysis cannot be used for chemical quantities such as a mole i.e it has no dimensional formula.

Read more on dimensional analysis here: brainly.com/question/24514347

5 0
2 years ago
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