When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Answer:
cell walls made of chitin
Explanation:
Fungal cells differ from mammalian cells in that they have cell walls that are composed of chitin, glucans, mannans, and glycoproteins. Both mammalian and fungal cells have cell membranes; however, they differ in their lipid composition.
The grassland preserve, without the lion pride, will have an increase on antelope and zebra, therefore decreasing the amount vegetation as they eat the vegetation, without a control in the population, this could be potentially dangerous for the preserve
