1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Oksi-84 [34.3K]
3 years ago
15

How to determine the enzymes used to cut plasmid in cloning.

Biology
1 answer:
Alex73 [517]3 years ago
8 0
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
You might be interested in
In the mouse, gene B can produce black pigment from a colorless precursor molecule. A mouse having at least one B allele can pro
Lyrx [107]

Answer:

Black

Explanation:

In the mouse a certain colorless pigment precursor exists.

  • If the B allele is present: precursor is converted into black pigment.
  • If the genotype is bb: albino

In addition:

  • If the A allele is present: black pigment is converted to brown pigment
  • If the genotype is aa: black pigment is not converted and mouse remains black

If the mouse has a genotype aaBB, the colorless pigment is converted to black (because there is at least one B allele) but the black pigment cannot be converted into brown (because there's no A allele), so the mouse will have black fur,

3 0
3 years ago
Read 2 more answers
What is the scientific method? Is there just one universal scientific method? Why or why not?
MAXImum [283]

Answer:

"The scientific method describes the processes by which scientists gain knowledge about the world. It's characterized by six key elements: questions, hypotheses, experiments, observations, analyses, and conclusions. These elements are interrelated steps, so they don't always function in the same order."

Explanation:

google

8 0
3 years ago
Explain how women's right's were impacted by getting married in many states in the 1800's.
kodGreya [7K]

Answer:

In the early nineteenth century, married women in the US were legally subordinate to their husbands. Wives could not own their own property, keep their own wages, or enter into contracts. Beginning in 1839, states slowly began to enact Married Women’s Property Acts to allow women more control over their property and finances

5 0
2 years ago
Assuming that it will rain when the sky is cloudy and dark is an example of a fact or a prediction
Igoryamba
The best answer would be a prediction
8 0
3 years ago
Explain how to identify<br> a starting position on a line.
jolli1 [7]

Answer:

We can easily find out the beginning point of the line by using dot representation. When it comes to position vector, it expresses the exact position of certain object from the starting point of the coordinate system. The vector is a straight line that has a certain end which is fixed to its body.

3 0
3 years ago
Other questions:
  •  _____ are different forms of the same gene that are always found on the same position of two homologous chromosomes
    11·1 answer
  • Which of the following are the components of a neuron brain, spinal cord, and vertebral column dendrite, axon, and cell body sen
    9·1 answer
  • Wheat varieties are usually purelines because Group of answer choices there is little available genetic variation in wheat germp
    12·1 answer
  • What is dna in biology​
    5·2 answers
  • What do we call structures that are inherited from ancestors but have lost much or all of their original function due to differe
    5·1 answer
  • DNA is a double-stranded nucleic acid formed by millions of linked nucleotides that each contain the sugar deoxyribose, a
    5·1 answer
  • A scientist is examining a pedigree that includes several generations of an organism with XX/XY chromosome sex determination. Wh
    11·1 answer
  • The ______ pump blood away from the heart.
    11·2 answers
  • Prior to the let-down reflex, the mammary glands secrete a yellowish fluid called ________ that has less lactose than milk and a
    13·2 answers
  • Please help...<br>in most stems,the epidermis has pores called..........​
    14·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!