1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Oksi-84 [34.3K]
3 years ago
15

How to determine the enzymes used to cut plasmid in cloning.

Biology
1 answer:
Alex73 [517]3 years ago
8 0
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
You might be interested in
Cytoplasmic division or ____________ begins during anaphase of mitosis and continues as the cell pinches into two new cells.
vova2212 [387]

Cytoplasmic division or cell division begins during anaphase of mitosis and continues as the cell pinches into two new cells.

During  the proces of cytokinesis, the cell membrane pinches in at the cell equator, which forming the cleft called the cleavage furrow. The position which depends on the position of the astral and interpolar microtubules during the anaphase.During the process of anaphase, each pair of chromosomes is separated into two identical, and independent chromosomes.

The chromosomes which are separated by a structure that is known as the mitotic spindle.The cohesion proteins located between the sister chromatids disappear and  the sister chromatids which located at the equator of the metaphase plate Anaphase where is starts,and  separate and begin their migration toward the opposite poles of the mitotic spindle.

To learn more about cell division here

brainly.com/question/13312481

#SPJ4

5 0
2 years ago
More people have been to space than to the deepest level of the ocean
ziro4ka [17]

Answer:

True, 571 people have been to space, but only 4 have been to the bottom of the mariana trench( which is the deepest level of the ocean) also sub to Goated Bois on yt for irl content. Tell us what you think of our vids in the commets. Thanks so much.

Explanation:

7 0
2 years ago
A child climbs the stairs to the top of a waterslide and makes her way down. Which of the following best describes this in energ
Roman55 [17]
First the child gain potential energy as he climbs up the stairs of the waterslide. when the child starts to slides down in the waterslide, the potential energy he gains in climbing up is now converted to kinetic energy, because the child is already moving
5 0
3 years ago
How did the invention of agriculture affect human birth and death rates? How did it affect human population growth?
NikAS [45]
Women who were agriculturist worked hard and didn't take care of themselves even out in the heat that is one thing that affected death of them being pregnant and losing kids or them dying alone. Some men and women were separated and some couldn't be reached also because so many people were dying they had a difficult time repopulating.
6 0
3 years ago
Composition of water yam​
Anvisha [2.4K]
2 hydrogens and 1 oxygen
3 0
3 years ago
Other questions:
  • What does h.E.L.P stand for in health class 8th grade?
    9·2 answers
  • 27) The ducts that deliver bile and pancreatic juice from the liver and pancreas, respectively, unite to form the ________.
    13·1 answer
  • The basic building block of life is _____.
    5·2 answers
  • What would most likely happen if the number of shrimp decreased
    15·1 answer
  • What idea is Malthus known for?
    9·1 answer
  • Which of the following is not a physical property of iron? conductivity ability to rust color melting point
    5·1 answer
  • Define ecological pyramid and indicate the limitations of ecological pyramid​
    12·1 answer
  • How does the digestive system of poultry vary from other animals?
    14·1 answer
  • The four types of macromolecules in cells are nucleic_________<br> lipids, proteins, and_____
    14·2 answers
  • . hengityksen säätely
    5·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!