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Oksi-84 [34.3K]
2 years ago
15

How to determine the enzymes used to cut plasmid in cloning.

Biology
1 answer:
Alex73 [517]2 years ago
8 0
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
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How did the environment of Arabia serve as a form of protection during much of the region's history?
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Answer: A: Its fertile oases enabled farmers to grow enough food for themselves and for sale.

Explanation: The Arabia region is a region located within the Arabian desert. A desert is an area of extreme heat, often dry weather condition with barren lands that cannot support agriculture and food production. An oases on the other hand is a region of luscious, cultivate-able land within a desert.

Food is one of the most important basic needs of man and hardly can anyone survive without food for more than twenty days. Hence, the presence of oases within the otherwise dry environment of the Arabia region is a major protection against death from hunger.

Taking a closer look at other options, none of them guarantees food supply and income to the Arabia region unlike food production in oases. Hence, the fertile oases is really the most important factor that serves as protection in the region's history.

Correct option: A

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3 years ago
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Arturiano [62]

Answer:

B. Living organisms use matter and energy for their life processes

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Which of the following is an inference?
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5 0
2 years ago
Which person should the charge nurse assign as a roommate for a client diagnosed with aplastic anemia?
nikitadnepr [17]

Aplastic anemia refers to a disorder that takes place when the body loses the tendency to generate enough amount of new blood cells. Aplastic anemia is a rare and serious disorder that can develop or arise at any age. In the condition, the person becomes fatigued and possess a greater threat of developing infections and uncontrolled bleeding.  

Thus, in the given case, the nurse should appoint a roommate who is free from any kind of infectious disease or any other thing, which can stimulate an infection.  


7 0
3 years ago
Anyone wanna talk bc i'm hella bored
Afina-wow [57]

Answer:

same and hey

Explanation:

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