Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Nerve cells are functionally classified as sensory neurons, motor neurons, or interneurons. Sensory neurons (afferent neurons) are unipolar, bipolar, or multipolar shaped cells that conduct action potentials toward or into the central nervous system.
Answer:
The correct answer is d Lysosomes contain enzymes to breakdown damaged cellular material.
Explanation:
Lysosomes are called digestive organelle that contain many hydrolytic enzymes such as acid hydrolase ,peptidase, nuclease etc which aids in the digestion of various cellular components such as carbohydrates,protein,nucleic acid.
Lysosome perform the same function by the formation of auto phagolysosome which contain the those cellular molecules and organelles those are targeted for degradation.
The correct answer is A. Quantitative traits
Explanation:
In genetics, quantitative traits refer to traits or phenotypes (observable traits) that depend on many factors including multiple genes and actions in the environment. These traits differ from other traits because the traits tend to be more diverse in a population. In the case presented, the shell color in clams is an example of a quantitative trait because this phenotype is the result of three different each and each gene contains two alleles which means the color depends on many factors. Additionally, as most quantitative traits there is a higher variation in the phenotype considering clams can have different hues.
