I think you forgot to attach the options along with the question. I am answering the question based on my research and knowledge. the two factors that <span>affect an enzyme's operation the most are temperature and pH level. Enzymes function at at its best when pH is 7.4. I hope the answer helps you.</span>
The theme is the writer's view of or comment about the subject. The answer to your question is D. I hope this is the answer that you are looking for and it comes to your help.
D. This is because artificial selection is basically the human breeding of plants or animals, and we do so in a way to bring forth the best traits to solve a problem or create a better product. Hope this helps!