Question

You have identified a novel gene, Ums1, whose protein product is a component of the RISC in the nematode C. elegans. You believe this protein (like all the RISC proteins) is critical for the production of miRNAs, in particular ones that slice the mRNA for a gene called Trtn1. Describe how you would use RNAi in worm lysates to knockdown Ums1 and show that slicing of Trtn1 is inhibited without Ums1. Include details like what part of Ums1 you would target with siRNAs, how trigger RNA would be delivered to the worms, and how you assay for sliced or unsliced Trtn1 mRNA. Show what positive results would look like (probably with an illustration of an autorad or bar graphs).

Answer 1

I would give me an answer because I’m on a timer for my test