During stressful, frightening and challenging situations, the hypothalamic-pituitary-adrenal (HPA) axis stimulates the endocrine stress response which causes adrenaline rush. The part of brain called as amygdala processes the information about stressful condition and stimulates the hypothalamus which in turn establishes a contact with rest of the body through sympathetic nervous system. A hypothalamus transmits signal to adrenal glands via adrenal medulla which releases adrenaline into the bloodstream. This entire process takes some time and hence the adrenal rush is created after the stressful condition is over and hence maintains the dynamic equilibrium
The smallest fragments of DNA will be witnessed near the bottom of the gel, on the other hand, the heavier fragments will be visible at the top. The theory behind this is there is a specific pore size on the basis of the concentration of acrylamide/agarose in the gel.
