Nematodes are wormlike organisms which can be seen with naked eye, live in water-filled pore spaces in the soil. Nematodes are in large number in the upper soil layers where organic matter, plant roots, and other resources are most abundant.
The functions of nematodes:
- Free-living nematodes decompose organic material into nutrients and cycled them in the soil by feeding on some bacteria and fungi.
- Nematodes help in distributing bacteria and fungi through the soil and along roots by carrying live and dormant microbes.
- They used as food for higher predators, soil microorthropodes.
- They eat disease-causing organisms, thus suppress their growth.
- They acts as potential bio- control agents.
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Answer:
Primers should be:
3 'ACGTATGTCGCTTAGT 5'
5 'ACGTTCCAGTCAATGA 3'
Explanation:
DNA replication occurs with the need for the presence of primers that are a small sequence of nitrogenous bases that will allow the initiation of replication.
Primers are extremely important in PCR and are represented as small pieces of single-stranded DNA that specify the region of DNA that should be replicated during PCR. These primers attach to the single strand of DNA by pairing the nitrogenous bases allowing the DNA polymerase to recognize where replication begins.
Regarding the question above, the primers should be:
3 'ACGTATGTCGCTTAGT 5'
5 'ACGTTCCAGTCAATGA 3'
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