Bright cherry red color is produced upon the addition of methyl red indicator to a culture of a Gram negative bacilli in MR-VP broth if the organism used the mixed acid pathway of fermentation.
What is MR-VP test?
- As part of the IMViC testing, Methyl Red (MR) and Voges-Proskauer (VP) broth is utilized as the medium in which both the Methyl Red and Voges-Prosakuer tests can be conducted.
- It is a straightforward broth made up of dextrose or glucose, buffers, and peptone.
- In the Methyl Red test, an infected tube of MR-VP broth is mixed with the pH indicator methyl red.
- The acids will overwhelm the buffers in the medium and create an acidic environment if the organism employs the mixed acid fermentation pathway and creates stable acidic end products.
- If acidic end products are present when methyl red is introduced, the methyl red will remain red.
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