Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
B. trenches .......................
Answer:
identification
Explanation:
because identification means identifying, elaborating, exploring more about that field by identifying it.
This question is incomplete because the images a section 2 are not given. I searched for the complete question but could not find it. However, based on my knowledge and the characteristics of the tundra this is the answer.
The correct answer is C. The climate is harsh, which limits the number of species that can live there.
Explanation
The tundra is the name of an ecosystem that occupies a fifth of the earth's surface, mainly in the northern hemisphere, this is characterized by having a frozen subsoil that limits the emergence and growth of natural vegetation and trees. This ecosystem is the coldest on the planet due to its location near the poles, its landscape is characterized by a layer of snow that covers most of the ground. In addition, the climatic conditions are extreme, which limits the number of plant and animal species that can live there. According to the above, the correct answer is C. The climate is harsh, which limits the number of species that can live there.
