Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
The correct answer is A The student is investigating the passive transport of diffusion.
Explanation:
Diffusion is a type of transport process which helps in the transport of small molecules and gases towards their concentration gradient that"s means from region of high concentration to the low concentration region untill equilibrium is reached.
In this question the 10 drops are red coloured food particles that are placed into the beaker, diffuses to all the regions of the beaker untill the concentration of that red colored food particle become same in all the regions to which red colored food particle diffuse.
They have kingdom, class, and phylum, I’m kind of confused about the answer because of that.
