Question

Arrange the following in the proper sequence in which they occur during a single PCR cycle. 1. Addition of DNA nucleotides by Taq polymerase 2. Complementary base pairing between primers and target DNA 3. Heat separation of strands of target DNA

Answer 1

Answer:

3-2-1

Explanation:

PCR is the process of DNA duplication in vitro. We can make millions of copies of DNA in a very short period.

PCR completed in three steps;  as in question these are

Step 1: Denaturation; Heat separation of strands of target DNA.

Step 2: Annealing; as complementary base pairing between primers and target DNA.

Step 3: Extension; Addition of DNA nucleotides by Taq DNA polymerase.