1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
lana [24]
3 years ago
11

You are trying to determine information about the structure of a protein that you have purified. You carry out a series of exper

iments on this protein. The results are listed below.
1. Size exclusion (aka gel filtration) chromatography of the protein indicates an apparent molecular weight of 240,000 kDa.
2. SDS polyacrylamide gel electrophoresis of the protein carried out WITHOUT the addition of a disulfide-bond reducing agent shows the protein as having an apparent molecular weight of 60,000 kDa.
3. After treatment with performic acid, which cleaves disulfide bonds, the same technique (SDS PAGE) reveals two bands of molecular weights 34,000 kDa and 26,000 kDa.
4. N-terminus identification shows Ala and Leu as amino terminal residues, in equal amounts.

Describe what you can determine about the protein's structure from the above information by answering the questions below.

a. Molecular weight of the native functional protein.
b. Does the protein contain any disulfide bonds? If yes, can you estimate how many ?
c. Can you determine anything about any secondary structure of the protein? If yes, what?
d. Does the protein have quaternary structure? If yes, how many subunits, of what size?
Biology
1 answer:
aliya0001 [1]3 years ago
5 0

Answer:

if I am not draw molecular weight of the directive functional protein is your answer give thanks to me

You might be interested in
HELP I WILL GIVE BRAINLIEST
jek_recluse [69]

Explanation:

members of the same species battle each other to protect their young

5 0
3 years ago
Read 2 more answers
Which resource is a renewable resource?<br> A. Coal<br> B. Gemstones<br> C. Metal<br> D. Trees
valina [46]
The answer is d. tress
3 0
3 years ago
Read 2 more answers
16. Which of the following stimuli would cause the liver to produce more glycogen?
stepladder [879]
The answer should be C
6 0
2 years ago
Which statement best describes Mendel's principle of segregation?​
Zina [86]

Answer:

Each parent passes on only one factor for each trait to its offspring.

6 0
3 years ago
What are barrier islands? What protects the barrier islands?
dem82 [27]

Answer:

Barrier islands form in three ways. They can form from spits, from drowned dune ridges or from sand bars. Longshore drift is the movement of sand parallel to the shore caused by the angle of the waves breaking on the beach. When a storm such as a hurricane digs an inlet through the spit a barrier island is formed. Barrier islands are a specific type of island that lie parallel to the coastline of a larger mainland. They are separated from shore by a bay, lagoon or sound and because of where they are situated, they protect the coast from being directly impacted by storm waves or winds.

Explanation:

3 0
2 years ago
Other questions:
  • The process of what adds a phosphate group to adp creating atp?
    12·1 answer
  • Which of the following is an example of a beneficial mutation?
    11·2 answers
  • Diffusion is movement of a molecule from an area of higher concentration to an area of lower concentration. True or false
    5·1 answer
  • Miranda wants to become a nurse but she doesn't have any experience in the field what is one way she could improve her opportuni
    14·2 answers
  • About 90 percent of stars on the Hertzsprung-Russell (H-R) diagram are _____.
    14·2 answers
  • What is the life expectancy of white males and females in the United States?
    6·1 answer
  • Which of the following is true about protein molecules? A. Protein molecules are made up of strands of DNA joined together by am
    14·1 answer
  • A species of bird enters a desert environment, and is getting its water by pecking into the stems of cacti. Cacti with which of
    10·1 answer
  • State three reasons why children resemble their parents​
    6·2 answers
  • TEST III Name the techniques used in cooking foods​
    14·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!