The answer I think is the environment for this one. <span />
1. Our results showed that test tube B contained end products of protein digestion, meaning that the isolated enzyme has the ability to break down protein. The end products of protein digestion include small polypeptides, dipeptides, and amino acids. In general, test tube B will contain the building blocks of proteins, which are the amino acids.
2. The two test tubes were placed in a hot water bath at 37oC. This is because the enzyme studied is a human enzyme and it works best at the human body temperature, which is approximately 37oC.
Enzymes are biological catalysts, which means that they accelerate chemical reactions. As a result, an enzyme's activity is greatly affected by temperature. Every enzyme, depending on each function and origin, has a specific range of ideal temperature conditions. If the temperature rises above the maximum accepted, then the enzyme denatures and loses its structure.
3. If the enzyme studied was added in a tube containing starch instead of proteins, then nothing would happen. This is because the enzymes are highly specialized and have very specific actions. It has been proved that this enzyme's activity involves the digestion of proteins.
Starch is a polysaccharide produced by plants, this means that starch is a carbohydrate. Given that starch is not a protein, it will not be digested by the enzyme. An enzyme digesting proteins, will not have any effect on carbohydrates.
4. The smaller molecules which are the result of the digestion happening in the digestive system are absorbed by the human body. More specifically, the products of protein digestion are absorbed by the intestine and then transported into the bloodstream. From the blood, they are then absorbed by other tissues.
After they are absorbed, they are used to build muscles or other compounds which can produce energy.
If the sperm cell fertilises the ovum, and implantation does not occur, then the corpus luteum degenerates and it turns into corpus albicans, stopping progesterone secretion. If fertilisation occurs and implantation also, the syncytiotrophoblast, the "root part" of the blastocyst (one of the early structures originated from the zygote), produces a hormone that is called <span>human chorionic gonadotropin (hCG). The hCG is what makes the corpus luteum keep secreting progesterone and maintain itself. The progesterone will then help keep the endometrium and, therefore, allow the development of the embryo to happen.</span>
The presence of the minute growth near the disc signifies that the bacterial innoculum contains mutant microorganisms that are able to grow in the presence of toxic substances.
<h3>What are zones of inhibition?</h3>
The sensitivity testing in a microbiology laboratory is done to determine the effect of antibiotics on various bacteria organisms
The zone of inhibition when a disk method is used signifies the area of media where bacteria are unable to grow, due to presence of a drug that impedes their growth.
If there are presence of minute growth near the disc, it means that the bacterial innoculum contains mutant microorganisms that are able to grow in the presence of toxic substances such as disinfectants.
If these mutant bacteria organisms are not cleared by the disk method, it means that they cannot be killed by the disinfectant/antiseptics that is being tested.
Learn more about antiseptics here:
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